Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Neurod2

Cell type

Cell type Class
Neural
Cell type
Cortex
NA
NA

Attributes by original data submitter

Sample

source_name
NeuroD2 ChIP-SEQ from postnatal cortex
strain
BALB/c
developmental stage
P0 pup
tissue
postnatal cortex
chip antibody
NeuroD2 antibody (Abcam, ab104430, GR94291-1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissue was cross-linked by 10 min of 1% formaldehyde treatment. After IP, stringent washes, and proteinase K treatment, DNA was extracted by standard phenol-chloroform extraction and ethanol precipitation. Library was prepared for the single end read Illumina HiSeq 2500 platform by Genewiz, Inc.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
40604773
Reads aligned (%)
97.5
Duplicates removed (%)
13.5
Number of peaks
5023 (qval < 1E-05)

mm9

Number of total reads
40604773
Reads aligned (%)
97.3
Duplicates removed (%)
13.5
Number of peaks
4967 (qval < 1E-05)

Base call quality data from DBCLS SRA